Journal: Analytical Chemistry

Article Title: Evolution and Impact of Nucleic Acid Amplification Test (NAAT) for Diagnosis of Coronavirus Disease

doi: 10.1021/acs.analchem.3c05225

Figure Lengend Snippet: Schematic representation of (a) SARS-Cov-2 virion and its structural protein (b) spike protein denoting S1 and S2 subunit and its binding with ACE2 receptor. [Created with BioRender.com .]

Article Snippet: Different LoDs have been observed for the SARS-CoV-2 MassArray Test (0.69–2.75 copies/mL), Agena SARS-CoV-2 Panel (2.5 copies/mL), and Ethos Laboratories SARS-CoV-2 MALDI-TOF Assay (1 TCID50/mL), with PPA and NPA values for the three tests ranging from 95% to 100%.

Techniques: Binding Assay

Journal: Analytical Chemistry

Article Title: Evolution and Impact of Nucleic Acid Amplification Test (NAAT) for Diagnosis of Coronavirus Disease

doi: 10.1021/acs.analchem.3c05225

Figure Lengend Snippet: Schematic representation of the SARS-CoV-2 genome, which includes a single-stranded RNA having various Open Reading Frames (ORF1a, ORF1b, ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8a, ORF8b, ORF9b), leading to the formation of 16 nonstructural protein (nsps1–nsps16), structural proteins like S (spike protein) having the S1 subunit, which includes NTD, RBD, SD1, and SD2 and the S2 subunit, which contains FL, HR1, HR2, and TM. Other structural proteins are E (Envelop protein), M (Membrane protein), N (Nucleocapsid protein). PL-pro (papain-like protease), and 3CL-pro (cysteine proteases). [Created with BioRender.com .]

Article Snippet: Different LoDs have been observed for the SARS-CoV-2 MassArray Test (0.69–2.75 copies/mL), Agena SARS-CoV-2 Panel (2.5 copies/mL), and Ethos Laboratories SARS-CoV-2 MALDI-TOF Assay (1 TCID50/mL), with PPA and NPA values for the three tests ranging from 95% to 100%.

Techniques: Membrane

Journal: Diagnostics (Basel, Switzerland)

Article Title: Rapid Evaluation of the Xpert ® Xpress CoV-2 plus and Xpert ® Xpress CoV-2/Flu/RSV plus Tests.

doi: 10.3390/diagnostics13010034

Figure Lengend Snippet: Figure 2. Regression analysis of the Xpert® Xpress SARS-CoV-2, Xpert® Xpress CoV-2 plus and Xpert® Xpress CoV-2/Flu/RSV tests, including the equation of the line and R2 values. (a) Xpert®

Article Snippet: These specimens were selected by COVID-19 wave and were previously genotyped by the AllPlex SARS-CoV-2 Variants I and II and NovaPlex SARS-CoV-2 Variants V tests (all Seegene, Seoul, Republic of Korea) and/or TaqMan SARS-CoV-2 Mutation Panels (Thermo Fisher Scientific, Waltham, MA, USA) and/or MassARRAY SARS-CoV-2 Variant Panel v3 (RUO) (MassARRAY; Agena Biosciences, San Diego, CA, USA), or by next generation sequencing performed at the NICD.

Techniques: